1. CS AS Roma (BT) – Rapid CFR Suceava (SV) 1-3
2. Rapid Dumeşti (IS) – CFR Paşcani (IS) 2-0
3. FC Ceahlăul 2 P.Neamţ (NT) – FC Laminorul Roman (NT) 3-0
4. FC Girceni 2008 (VS) – FC Luceafărul Lotus Felix (NT) 3-0
5. FC Stelele Vrancei – FC Panciu (VN) 1-5
6. FC Willy 2 Bacău – FC Willy Bacău 4-6
7. CS Politehnica Galaţi – FC Oţelul 2 Galaţi 2-1
8. CF Brăila – Arrubium Macin (TL) 8-0
9. CS Viitorul Însurăţei (BR) – FC Petrolul ZF 1948 Berca (BZ) 0-0, 4-1 (pen)
10. AS Municipal Constanţa – CSM Medgidia (CT) 3-0
11. CS Eforie (CT) – SC FC Viitorul Constanţa SA *
12. CS Abatorul Slobozia (IL) – FC Chimia Brazi (PH) 1-3
13. Petr.Kaproni Boldeşti (PH) – Conpet Ploieşti (PH) 3-2
14. FC Dunărea Călăraşi – SC FC Prefab Modelu (CL) 3-0
15. CS Inter Clinceni (IF) – Victoria Adunaţii Copăceni (GR) 5-1
16. FC Rapid Bucureşti 2 – SC FC Progresul Bucureşti SA 3-0
17. Comprest GIM Bucureşti – Juventus Bucureşti 0-4
18. CS Petrolul Videle (TR) – CS Alro Slatina (OT) 1-0
19. CS Gaz Metan Finta (DB) – Electrosid Titu (DB) 1-3
20. FC Argeş Piteşti SA 2 (AG) – SC FCM Târgovişte SA (DB) 2-2, 3-4 (pen)
21. FC Argeş Piteşti SA 3 (AG) – Oltchim Rm.Vâlcea (VL) 0-4
22. CS Prometeu Craiova (DJ) – FC Caracal (OT) 1-0
23. Triumf Bârca (DJ) – FC Progresul Corabia (DJ) 2-2, 3-2 (prel)
24. CS Ralbex Turcineşti (GJ) – Min.Mehedinţi Valea Copcii (MH) 0-2
25. CS Min.Jilţ Mătăsari (GJ) – CS Jiul Rovinari (GJ) 1-1, 5-4
26. CS Minerul Anina (CS) – CS Dacia Orăştie (HD) 3-0
27. FC Zlatna (AB) – FC Marmosim Simeria (HD) 1-0
28. FC Progresul Gătaia (TM) – FC CFR Timişoara 0-2
29. CS Nuova Mamma Mia Becicherecu Mic –Viitorul Sânandrei (TM) 2-1
30. AS Pro Nădlac (AR) – FC Timişoara SA II 0-7
31. Gloria CTP Arad – ACU V.Goldiş Arad 6-1
32. ENA Inter Făgăraş (BV) – FC Cisnădie (SB) 3-2
33. FC Râşnov (BV) – Royal Braşov 2-1
34. CF Predeal (BV) – Forex Braşov 3-0
35. ASA Miercurea Ciuc (HR) – Unirea Ungheni (MS) 1-3
36. AS Vetrix Satu Lung (MM) – FC Gloria Bistriţa 2 (BN) 0-2
37. CS Arieşul Turda 2 (CJ) – Unirea Dej (CJ) 1-3
38. CS Sănătatea SP Cluj – CS Mechel Câmpia Turzii (CJ) 3-1
39. FC Bihor Oradea 2 (BH) – CF Zalău (SJ) 1-2
40. CF Liberty Oradea 2 (BH) – FC Bihorul Beiuş (BH) 0-3
*se disputa pe 5 august 2009
